Healthier kids, brighter futures

Research

Publications

Embryology Unit

2013 - 2016

  1. Fan X, Loebel DAF, Bildsoe H, Wilkie EE, Qing J, Wang J and Tam PPL (2016) Tissue interactions, cell signaling and transcriptional control in the cranial mesoderm during craniofacial development. AIMS Genetics 3(1):74-98 Doi:10.3934/genet.2016.1.74 (accepted 5/4/2016; e-pub 11/4/2016) 
  2. Afanassieff M, Osteil P, Savatier P. (2015) Generation of Embryonic Stem Cells in Rabbits. Methods Mol Biol. 
  3. Fossat N, Ip CK, Jones VJ, Studdert JB, Khoo P-L, Lewis SL, Power P, Tourle K, Loebel DAF, Kwan KM, Behringer RR and Tam PPL (2015)  Context-specific function of LIM Homeobox 1 (LHX1) transcription factor in head formation of the mouse embryo. Development 
  4. McShane SG, Molè M, Savery D, Greene NDE, Tam PPL and Copp AJ (2015) Cellular basis of neuroepithelial bending during mouse spinal neural tube closure. Dev Biol 
  5. Kondo MA, Gray LJ, Pelka GJ, Leung S-K, Christodoulou J, Tam PPL and Hannan AJ (2015) Affective dysfunction in a mouse model of Rett syndrome: Therapeutic effects of environmental stimulation and physical activity. Dev Neurobiol  
  6. Greenlees R*, Mihelec M*, Yousoof S*, Speidel D, Wu SK, Rinkwitz S, Prokudin I, Perveen R, Cheng A, Ma A, Nash B, Gillespie R, Loebel DAF, Clayton-Smith J, Lloyd IC, Grigg JR, Tam PPL, Yap AS, Becker TS, Black GCM, Semina E, Jamieson RV (2015)  Mutations in SIPA1L3 cause eye defects through disruption of cell polarity and cytoskeleton organization. Human Molecular Genetics (*equal first authors)
  7. Schmaltz-Panneau, B., Jouneau, L., Osteil, P., Tapponnier, Y., Afanassieff, M., Moroldo, M., Jouneau, A., Daniel, N., Archilla, C., Savatier, P. and Duranthon, V. (2014) Contrasting transcriptome landscapes of rabbit pluripotent stem cells in vitro and in vivo.  Anim Reprod Sci 149(1-2):67-79.
  8. Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, Mure M, Afanassieff M, Cleroux E, Guibert S, Chen J, Vallot C, Acloque H, Genthon C, Donnadieu C, De Vos J, Sanlaville D,  Guérin JF , Weber M, Stanton L, Rougeulle C, Pain B, Bourillot PY, and Savatier P (2015) Reinforcement of STAT3 activity reprograms human embryonic stem cells towards naïve-like pluripotency.  Nat Comm
  9. Gold WA, Williamson SL, Kaur S, Hargreaves IP, Land JM, Pelka GJ, Tam PPL and Christodoulou J (2014) Mitochondrial dysfunction in skeletal muscle of a mouse model of Rett Syndrome (RTT): Implications for the disease phenotype. Mitochondrion 
  10. Yap, C.*, Goh,H.W.,*, Familari,M., Rathjen,P.D. & Rathjen, J. (2014). The formation of proximal and distal definitive endoderm populations in culture requires p38 MAPK activity. J Cell Science (*authors contributed equally to this publication)
  11. Loebel DAF, Hor ACC, Bildose HK, Tam PPL. (2014) Timed deletion of Twist1 in the limb bud reveals age-specific impacts on autopod and zeugopod patterning. PLoS ONE 
  12. Fossat N, Tourle K, Radziewic T, Barrat K, Liebhold D, Studdert J, Power M, Jones V, Loebel D, and Tam P. (2014)  C to U RNA editing mediated by APOBEC1 requires RNA Binding Protein RBM47. EMBO Report 
  13. Kojima Y, Tam OH and Tam PPL (2014)   Timing of developmental events in the early mouse embryo. Seminar in Cell and Developmental Biology
  14. Kaufman-Francis, F., Goh, H.W., Kojima, Y.,  Studdert, J.B., Jones, V., Power, M.D., Wilkie,  E., Teber, E., Loebel, D.A.F. and Tam, P.P.L. (2014).   Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo.  Phil. Trans. R. Soc. B 
  15. Hamada, H. and Tam, P.P.L. (2014) Mechanisms of left-right asymmetry and patterning: Driver, mediator and responder.  F1000Reports
  16. Ip, C.K., Fossat, N., Jones, V., Lamonerie, T. and Tam, P.P.L. (2014)  Head formation: OTX2 regulates Dkk1 and Lhx1 activity in the anterior mesendoderm.  Development
  17. Williamson S, Peter  GB, Ellaway C, Pelka G, Tam PPL and Christodoulou J. (2014) Deletion of protein tyrosine phosphatase non-receptor type 4 (PTPN4) in twins with a Rett syndrome like phenotype. European Journal of Human Genetics
  18. Fossat, N and Tam, P.P.L. (2014)   Re-editing the paradigm of Cytidine (C) to Uridine (U) RNA editing.  RNA Biology
  19. Tanaka SS, Nakane A, Yasuka YL, Terabayashi T, Abe T, Nakao K, Asashima M, Steiner KA, Tam PPL and Nishinakamura R.    (2013) Dullard/Ctdnep1 modulates WNT signalling activity for the formation of primordial germ cells in the mouse embryo. PLoS ONE 
  20. BioEssays:  Special Issue on “Stem Cell Research: Stem Cell Science and Regenerative Medicine”, edited by Patrick Tam and Martin Pera, Wiley-Blackwell (2013)
  21. Tam PPL and Pera M (2013) Editorial: Stem cell science and regenerative medicine.  Special issue on Stem Cell (ed. PPL Tam and M Pera), Bioessays
  22. Mammalian Development: Molecules, Signals, and Morphogenetic Processes 
    Edited by Patrick PL Tam, W James Nelson and Janet Rossant. Cold Spring Harbor Laboratory Press. 
  23. Arkell RM, Fossat N and Tam PPL (2013) Wnt signalling in mouse gastrulation and anterior development: new players in the pathway and signal output. Current Opinion in Genetics and Development
  24. Loebel D A F, Radziewic T, Power M, Studdert J B and Tam P P L Generation of mouse embryos with small hairpin RNA-mediated knockdown of gene expression   Mouse Molecular Embryology, Methods in Molecular Biology Series  Edited by Mark Lewandoski, Humana Press-Springer
  25. Tanaka S, Yamaguchi Y, Jones, V J and Tam P P L  Analyzing gene function in whole mouse embryo and fetal organ in vitro.  Mouse Molecular Embryology, Methods in Molecular Biology Series Edited by Mark Lewandoski, Humana Press-Springer
  26. Funnell AP, Mak KS, Twine NA, Pelka GJ, Norton LJ, Radziewic T, Power M, Wilkins MR, Bell-Anderson KS, Perkins AC, Tam PPL, Pearson RC and Crossley M (2013)  Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing.  Molecular Cell Biology
  27. Loebel DAF, Kaufman-Francis K, Kojima Y, Chung HCY and Tam PPL (2013) Endoderm development in the mouse: From progenitors to organ buds. Translational Gastroenterology: Organogenesis to Disease.  Edited by Deborah L. Gumucio, Linda Samuelson and Jason Spence, Wylie
  28. Ng HH and Tam PPL (2013) The mystique of cellular reprogramming. Editorial Overview Current Opinion in Genetics and Development, Special issue on Cellular Reprogramming, edited by HH Ng and PPL Tam, Elsevier 
  29. Maxwell SS, Pelka GJ, Tam PPL and El-Osta A (2013)  Chromatin context and ncRNA highlight physiological targets of MeCP2 in brain.  RNA Biology
  30. Teddy Fauquier, Fabrice Chatonnet, Frédéric Picou, Sabine Richard, Nicolas Fossat, Nadine Aguilera, Thomas Lamonerie and Frédéric Flamant (2013) Purkinje cells and Bergmann glia are primary targets of the TRα1 thyroid hormone receptor during mouse cerebellum post-natal development. Development 

  31.  

Recent Publications 

  1. Chromatin context and ncRNA highlight targets of MeCP2 in brain. Maxwell SS, Pelka GJ, Tam PP, El-Osta A. RNA Biol. 2013 Oct 30;10(11). [Epub ahead of print] PMID: 24270455
  2. The Transcriptional and Functional Properties of Mouse Epiblast Stem Cells Resemble the Anterior Primitive Streak. Kojima Y, Kaufman-Francis K, Studdert JB, Steiner KA, Power MD, Loebel DA, Jones V, Hor A, de Alencastro G, Logan GJ, Teber ET, Tam OH, Stutz MD, Alexander IE, Pickett HA, Tam PP. Cell Stem Cell. 2013 Oct 15. doi:pii: S1934-5909(13)00414-1. 10.1016/j.stem.2013.09.014. [Epub ahead of print] PMID: 24139757
  3. Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing. Funnell AP, Mak KS, Twine NA, Pelka GJ, Norton LJ, Radziewic T, Power M, Wilkins MR, Bell-Anderson KS, Fraser ST, Perkins AC, Tam PP, Pearson RC, Crossley M. Mol Cell Biol. 2013 Aug;33(15):2976-87. doi: 10.1128/MCB.00074-13. Epub 2013 May 28. PMID: 23716600
  4. Dullard/Ctdnep1 modulates WNT signalling activity for the formation of primordial germ cells in the mouse embryo. Tanaka SS, Nakane A, Yamaguchi YL, Terabayashi T, Abe T, Nakao K, Asashima M, Steiner KA, Tam PP, Nishinakamura R. PLoS One. 2013;8(3):e57428. doi: 10.1371/journal.pone.0057428. Epub 2013 Mar 4. PMID: 23469192
  5. Alternative lengthening of telomeres in normal mammalian somatic cells. Neumann AA, Watson CM, Noble JR, Pickett HA, Tam PP, Reddel RR. Genes Dev. 2013 Jan 1;27(1):18-23. doi: 10.1101/gad.205062.112. PMID: 23307865
  6. The mesenchymal architecture of the cranial mesoderm of mouse embryos is disrupted by the loss of Twist1 function. Bildsoe H, Loebel DA, Jones VJ, Hor AC, Braithwaite AW, Chen YT, Behringer RR, Tam PP. Dev Biol. 2013 Feb 15;374(2):295-307. doi: 10.1016/j.ydbio.2012.12.004. Epub 2012 Dec 19. PMID: 23261931
  7. Initiating head development in mouse embryos: integrating signalling and transcriptional activity. Arkell RM, Tam PP. Open Biol. 2012 Mar;2(3):120030. doi: 10.1098/rsob.120030. PMID: 22754658
  8. Rho GTPases in endoderm development and differentiation. Loebel DA, Tam PP. Small GTPases. 2012 Jan-Mar;3(1):40-4. doi: 10.4161/sgtp.18820. PMID: 22714416
  9. Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton. Loebel DA, Hor AC, Bildsoe H, Jones V, Chen YT, Behringer RR, Tam PP. Dev Biol. 2012 Feb 15;362(2):132-40. doi: 10.1016/j.ydbio.2011.11.020. Epub 2011 Dec 8. PMID: 22178153
     

Key Publications

  1. Context-specific function of the LIM homeobox 1 transcription factor in head formation of the mouse embryo. Fossat N1, Ip CK2, Jones VJ3, Studdert JB3, Khoo PL3, Lewis SL3, Power M3, Tourle K3, Loebel DA2, Kwan KM4, Behringer RR4, Tam PP2. Development 2015, 142 (11):2069-79      F1000 Prime Recommended
  2. The formation of mesodermal tissues in the mouse embryo during gastrulation and early organogenesis. Tam PPL and Beddington RSP. Development 1987, 99: 109-126. PMID: 3652985
  3. Anterior patterning by synergistic activity of the early gastrula organizer and the anterior germ layer tissues of the mouse embryo. Tam PPL and Steiner KS. Development 1999, 126: 5171-5179. PMID: 10529433
  4. Conserved requirement of Lim1 function for cell movements during gastrulation. Hukriede NA, Tsang TE, Habas R, Khoo PL, Steiner K, Weeks DL, Tam PPL and Dawid IB. Dev. Cell 2003, 4: 83-94. PMID: 12530965
  5. Dkk1 and Wnt3 interact to control head morphogenesis in the mouse. Lewis SL, Khoo PL, De Young RA, Steiner K, Wilcock C, Mukhopadhyay M, Westphal H, Jamieson RV, Robb L and Tam PP. Development 2008, 135: 1791-801. PMID: 18403408
  6. IFITM/Mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion.Tanaka SS, Yamaguchi YL, Tsoi B, Lickert H, Tam PP. Dev Cell. 2005 Dec;9(6):745-56. PMID:16326387
     

Review Articles

  1. The mystique of cellular reprogramming. Ng HH, Tam PP. Curr Opin Genet Dev. 2013 Oct;23(5):501-3. doi: 10.1016/j.gde.2013.08.001. Epub 2013 Sep 1. PMID: 24004469
  2. Stem cell science and regenerative medicine. Tam PP, Pera MF. Bioessays. 2013 Mar;35(3):147-8. doi: 10.1002/bies.201300003. PMID: 23401027
  3. Intercellular interactions, position, and polarity in establishing blastocyst cell lineages and embryonic axes. Stephenson RO, Rossant J, Tam PP. Cold Spring Harb Perspect Biol. 2012 Nov 1;4(11). doi:pii: a008235. 10.1101/cshperspect.a008235. Review. PMID: 23125013
  4. Wnt signalling in mouse gastrulation and anterior development: new players in the pathway and signal output. Arkell RM, Fossat N, Tam PP. Curr Opin Genet Dev. 2013 Aug;23(4):454-60. doi: 10.1016/j.gde.2013.03.001. Epub 2013 Apr 19. PMID: 23608663
  5. Extrinsic regulation of pluripotent stem cells. Pera MF, Tam PP. Nature 2010, 465 (7299): 713-20. PMID: 20535200
  6. Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse. Rossant J, Tam PP. Development 2009, 136: 701-13. PMID: 19201946